Dilution characterstics of Coomasie blue in man.

The azo dye Coomassie Blue is relatively non-toxic, has a rapid disappearance rate, and does not cause any skin discoloration (Taylor and Shillingford, 1959). Because it has these properties the dye can be given repeatedly to man at short intervals of time, a particularly important quality for investigations based upon the Stewart-Hamilton indicator dilution curve. Indeed, the dye has already been successfully used in studies of cardiac output (Taylor and Shillingford, 1959) and of congenital heart defects (Oakley et al., 1960). This paper presents further information about the dilution characteristics of the dye in man. The results reported include procedures in which radioiodinated human serum albumin (1131 H.S.A.) and Coomassie Blue were injected simultaneously.